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KMID : 0380020060210030181
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Korean Journal of Biotechnology and Bioengineering
2006 Volume.21 No. 3 p.181 ~ p.187
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Effects of pH Control Methods on Removal Efficiency in Electrokinetic Bioremediation of Phenanthrene-contaminated Soil
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Kim Sang-Joon
Park Ji-Yeon
Lee You-Jin
Yang Ji-Won
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Abstract
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In this study, problems related with pH control in electrokinetic (EK) bioremediation of phenanthrene contaminated soil were observed, and the effects of pH control methods on the removal efficiency were investigated to search a further application strategy. In a preliminary experiment, it was found out by flask cultivation that a certain sulfate concentration was needed to degrade phenanthrene well using Sphingomonas sp. 3Y. However, when MgSO4 was used as sulfate source in EK bioremediation, the bacterial activity reduced seriously due to the abrupt decrease of pHs in soil and bioreactor by the combination of magnesium and hydroxyl ions. When another strong buffering compound was used to control the pH problem, the good maintenance of the bacterial activity and pHs could be observed, but the removal efficiency decreased largely. When a low concentration of MgSO4 was added, the removal efficiency decreased somewhat in spite of the good maintenance of neutral pHs. With the addition of NaOH as a neutralizing agent, the removal efficiency also decreased because of the increase of soil pH. Consequently the selection of electrolyte composition was a very important factor in EK bioremediation and some sulfate sources suitable for both bacterial activity and contaminant degradation should be investigated.
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KEYWORD
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pH control, electrokinetic bioremediation, phenanthrene, bacterial activity, electrolyte composition
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